Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments     

Gozari Mohsen  Bahador Nim  Jassbi Amir Rez  Mortazavi Mohammad Seddiq  Hamzehei Sam  Eftekhar Ebrahim 《海洋学报(英文版)》2019,38(12):84-90

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.  相似文献   

碎冰条件下单点系泊船舶动态响应特性研究     

杨佳东  吕海宁  王晋 《海洋工程》2019,37(2):29-39

为了研究不同单点系泊位置对极地系泊船舶在来冰角度改变时动态响应的影响,运用离散元方法(DEM),计算了船舶在60%密集度下,冰速为1.0 m/s、冰厚为1 m时不同角度的船冰阻力,结合系泊分析软件二次开发功能,计算分析系泊船舶在不同来冰角度和不同系泊位置下受上述冰情影响下的响应。通过对模拟结果与试验结果的对比,认为离散元方法(DEM)的数值模拟结果与试验结果吻合较好;单点系泊位置为距船头L/4时,系泊船舶能以最快速、最稳定的方式对来冰方向的变化做出响应。  相似文献   

中国物理海洋学研究70年:发展历程、学术成就概览   总被引：2，自引：2，他引：0  

魏泽勋  郑全安  杨永增  刘克修  徐腾飞  王凡  胡石建  谢玲玲  李元龙  杜岩  周磊  林霄沛  胡建宇  朱建荣  李均益  张正光  侯一筠  刘泽  田纪伟  黄晓冬  管玉平  刘志宇  杨庆轩  赵玮  宋振亚  刘海龙  董昌明  于卫东  连涛  陈朝晖  史久新  雷瑞波  刘煜  于福江  尹宝树  陈戈  王岩峰  李整林  熊学军  汪嘉宁  李晓峰  王永刚 《海洋学报》2019,41(10):23-64

本文概略评述新中国成立70年来物理海洋学各分支研究领域的发展历程和若干学术成就。中国物理海洋学研究起步于海浪、潮汐、近海环流与水团，以及以风暴潮为主的海洋气象灾害的研究。随着国力的增强，研究领域不断拓展，涌现了大量具有广泛影响力的研究成果，其中包括:提出了被国际广泛采用的“普遍风浪谱”和“涌浪谱”，发展了第三代海浪数值模式；提出了“准调和分析方法”和“潮汐潮流永久预报”等潮汐潮流的分析和预报方法；发现并命名了“棉兰老潜流”，揭示了东海黑潮的多核结构及其多尺度变异机理等，系统描述了太平洋西边界流系；提出了印度尼西亚贯穿流的南海分支(或称南海贯穿流)；不断完善了中国近海陆架环流系统，在南海环流、黑潮及其分支、台湾暖流、闽浙沿岸流、黄海冷水团环流、黄海暖流、渤海环流，以及陆架波方面均取得了深刻的认识；从大气桥和海洋桥两个方面对太平洋–印度洋–大西洋洋际相互作用进行了系统的总结；发展了浅海水团的研究方法，基本摸清了中国近海水团的分布和消长特征与机制，在大洋和极地水团分布及运动研究方面也做出了重要贡献；阐明了南海中尺度涡的宏观特征和生成机制，揭示了中尺度涡的三维结构，定量评估了其全球物质与能量输运能力；基本摸清了中国近海海洋锋的空间分布和季节变化特征，提出了地形、正压不稳定和斜压不稳定等锋面动力学机制；构建了“南海内波潜标观测网”，实现了对内波生成–演变–消亡全过程机理的系统认识；发展了湍流的剪切不稳定理论，提出了海流“边缘不稳定”的概念，开发了海洋湍流模式，提出了湍流混合参数化的新方法等；在海洋内部混合机制和能量来源方面取得了新的认识，并阐述了混合对海洋深层环流、营养物质输运等过程的影响；研发了全球浪–潮–流耦合模式，推出一系列海洋与气候模式；发展了可同化主要海洋观测数据的海洋数据同化系统和用于ENSO预报的耦合同化系统；建立了达到国际水准的非地转(水槽/水池)和地转(旋转平台)物理模 型实验平台；发展了ENSO预报的误差分析方法，建立了海洋和气候系统年代际变化的理论体系，揭示了中深层海洋对全球气候变化的响应；初步建成了中国近海海洋观测网；持续开展南北极调查研究；建立了台风、风暴潮、巨浪和海啸的业务化预报系统，为中国气象减灾提供保障；突破了国外的海洋技术封锁，研发了万米水深的深水水听器和海洋光学特性系列测量仪器；建立了溢油、危险化学品漂移扩散等预测模型，为伴随海洋资源开发所带来的风险事故的应急处理和预警预报提供科学支撑。文中引用的大量学术成果文献(每位第一作者优选不超过3篇)显示，经过70年的发展，中国物理海洋学研究培养了一支实力雄厚的科研队伍，这是最宝贵的成果。这支队伍必将成为中国物理海洋学研究攀登新高峰的主力军。  相似文献   

桂林-阳朔地区DEM地形特征与岩性相关性分析及分类研究     

陈霄燕  潘军  邢立新  蒋立军  孙也涵  仲伟敬  范博文 《地球信息科学学报》2019,21(12):1867-1876

地形地貌是岩性解译的重要信息,地形因子作为描述DEM数字曲面几何特征的定量指标参数,可用来定量化表达不同岩性所在地区地形地貌特征。本文以桂林-阳朔地区为研究区,研究地形因子数学、地质意义,建立岩性与地形因子组合间的定量关联,进而实现岩石类型划分。本文基于ASTERGDEM提取坡度、起伏度等12个地形因子,在分析各个地形因子地质意义基础上,通过聚类分析及方差分析的多元统计分析方法,研究各岩性地形因子特性及其关联性,建立研究区岩性之间的定量差异;此外,利用因子分析方法研究岩性分类过程中的主导因素,确定适宜岩性分类方法以实现定量化岩性分类。实验结果表明：不同岩性、不同地形地貌的地形因子（组合）之间具有显著差异,基于因子分析得到的宏观地形复杂度指数（MTI）以及微观曲率指数（MCI）对岩石类型的分类精度达77.36%。研究表明,地形复杂度等地形因子可用于岩性分类,采用因子分析方法可获取反映地形地貌宏观、微观特征的定量指标,且岩性分类效果良好。  相似文献   

The effectiveness of a pseudo-inverse extended Born operator to handle lateral heterogeneity for imaging and velocity analysis applications     

Abdullah Alali  Bingbing Sun  Tariq Alkhalifah 《Geophysical Prospecting》2020,68(4):1154-1166

Wave equation–based migration velocity analysis techniques aim to construct a kinematically accurate velocity model for imaging or as an initial model for full waveform inversion applications. The most popular wave equation–based migration velocity analysis method is differential semblance optimization, where the velocity model is iteratively updated by minimizing the unfocused energy in an extended image volume. However, differential semblance optimization suffers from artefacts, courtesy of the adjoint operator used in imaging, leading to poor convergence. Recent findings show that true amplitude imaging plays a significant role in enhancing the differential semblance optimization's gradient and reducing the artefacts. Here, we focus on a pseudo-inverse operator to the horizontally extended Born as a true amplitude imaging operator. For laterally inhomogeneous models, the operator required a derivative with respect to a vertical shift. Extending the image vertically to evaluate such a derivative is costly and impractical. The inverse operator can be simplified in laterally homogeneous models. We derive an extension of the approach to apply the full inverse formula and evaluate the derivative efficiently. We simplified the implementation by applying the derivative to the imaging condition and utilize the relationship between the source and receiver wavefields and the vertical shift. Specifically, we verify the effectiveness of the approach using the Marmousi model and show that the term required for the lateral inhomogeneity treatment has a relatively small impact on the results for many cases. We then apply the operator in differential semblance optimization and invert for an accurate macro-velocity model, which can serve as an initial velocity model for full waveform inversion.  相似文献   

Numerical homogenization-based seismic assessment of an English-bond masonry prototype: Structural level application     

Luís C. Silva  Paulo B. Lourenço  Gabriele Milani 《地震工程与结构动力学》2020,49(9):841-862

A multiscale strategy is evaluated at a structural level for the analysis of unreinforced masonry structures. The mechanical characterization of the masonry is deduced from homogenization-based micro-scale finite element (FE) models. The derived data are here employed at a structural level via a discrete FE model. The discrete FE model is composed of quadrilateral rigid plates interconnected through vertical and horizontal interfaces. On the interfaces, between adjoining discrete elements, a model that accounts for the in- and out-of-plane behavior of masonry, with damage and plasticity, is adopted. Such interfaces represent the material pre- and post-peak regimes, its orthotropy, and, depending on the micro-model assumed, account by three-dimensional shear effects that are especially important for multi-leaf walls and complex regular textures. The discrete model has been implemented in an advanced structural analysis software where powerful built-in features as the arc-length method, line-search algorithm, and implicit or explicit solver schemes are available. The multi-scale model is applied for the dynamic study of a small English-bond masonry house prototype subjected to a series of consecutive earthquake records. Detailed comparisons between the experimental and numerical data are presented, including the results obtained through a continuous total strain rotating crack model. Quasi-static and dynamic analyses are conducted. Results demonstrate that when enough experimental information is available on the masonry components under tension, shear, and compression regimes, the approach predicts well the seismic structural response in terms of time-history displacements, seismic capacity, and damage patterns. The required computational cost (CPU time) is very attractive.  相似文献   

Perceptual models of uncertainty for socio-hydrological systems: a flood risk change example     

Ida K. Westerberg  Keith J. Beven  Gemma Coxon  Tobias Krueger 《水文科学杂志》2020,65(Z2):1705-1713

ABSTRACT

Characterizing, understanding and better estimating uncertainties are key concerns for drawing robust conclusions when analyzing changing socio-hydrological systems. Here we suggest developing a perceptual model of uncertainty that is complementary to the perceptual model of the socio-hydrological system and we provide an example application to flood risk change analysis. Such a perceptual model aims to make all relevant uncertainty sources – and different perceptions thereof – explicit in a structured way. It is a first step to assessing uncertainty in system outcomes that can help to prioritize research efforts and to structure dialogue and communication about uncertainty in interdisciplinary work.  相似文献   

2016年新疆呼图壁6.2级地震前b值异常特征研究   总被引：1，自引：0，他引：1       下载免费PDF全文

刘子璇  冯建刚  王维桐 《地震工程学报》2020,42(1):150-156

利用2009年以来中国地震台网中心地震编目资料,以2016年新疆呼图壁6.2级地震作为研究对象,采用最大似然法进行b值空间扫描计算,获取震前震中及其邻区地震b值的空间图像。研究结果显示,2016年呼图壁6.2级地震发生在震前显著低b值区域,该异常特征可能反映了地震孕育的应力积累过程,印证了b值的物理意义。  相似文献   

2019年长宁M_S6.0地震邻区分钟采样地电场分析     

王玮铭  谭大诚 《地震》2020,40(3):41-51

2019年6月17日四川宜宾市长宁县发生M_S6.0地震, 分析震中周边600 km内7个地电场台站观测分钟值数据, 获知震前2个月左右红格等6个台站地电场均存在不同方位之间、 同方位长短极距之间的相关系数持续降低, 6月17日这些相关系数基本降至最低, 反映出这6个场地的地电场在此次地震前后发生了非均匀变化; 同时, 这6个台站的地电场优势方位角出现了约45°或90°跃变, 并伴有显著发散或收窄现象。分析表明长宁M_S6.0地震前后, 这6个场地的多种地电场异常现象具有局部场地效应、 时间准同步性。  相似文献   

顾及地形起伏的无人机影像覆盖分析方法     

张衡  李骁  叶朋飞 《测绘通报》2020,(1):102-106

使用无人机实施测绘航空摄影时，由于无人机相对航高较低，地面起伏会对无人机影像的分辨率、覆盖范围、重叠度造成较大的影响，影像成果会出现分辨率不足、重叠度不够、覆盖漏洞等缺陷。针对这一情况，本文提出了一种利用数字微分正解法的计算方法，借助DEM准确计算每张影像的覆盖范围，并使用FME软件高效生成全部影像的覆盖范围。经过实际使用，验证了该方法可以在航线设计阶段准确预测并分析全部影像的覆盖范围、重叠度，因此可及时发现设计问题并调整航线。该方法可以有效减少因地形起伏造成的影像覆盖缺陷，减少返工现象，从整体上提高了作业效率。  相似文献